| 英文摘要: |
Abstract Recombinant sortase A (SrtA) was used to
immune rabbit, and the inhibitory activity of anti-SrtA
serum on Staphylococcus aureus bioWlm formation was
tested. BioWlm formation was inhibited by anti-SrtA rabbit
serum in S. aureus ATCC25923 and two clinical isolated
strains. The antiserum was separated into two fractions, and
the main component with the inhibitory activity was dem-
onstrated to be the IgG fraction. Two proteins interact with
the IgG fraction were identiWed by using an in vitro pull-
down assay and were conWrmed to be lipase 2 and -hemolysin
by mass spectrometry. Cross-interaction between SrtA and
lipase 2 was further conWrmed by Western blotting. Addi-
tion of anti-lipase 2 serum in the culture medium also
showed inhibitory eVect against bioWlm formation.
Together, our study suggests anti-SrtA serum inhibits
S. aureus bioWlm formation and lipase 2 is one of the tar-
gets of anti-SrtA serum in this inhibition process. This is
the Wrst study to demonstrate the roles of antisera against
SrtA and lipase 2 in the inhibition of bioWlm formation in
S. aureus. |
