| 英文摘要: |
Abstract Recombinant proteins bearing one (sM2)
or three (3sM2) copies of M2 epitope from influenza
virus were expressed in Escherichia coli. Mice were
administrated with these two proteins and systemic
and mucosal immune responses were analyzed. Com-
pared with sM2, 3sM2 inducedM2-specific serumIgG
and mucosal IgA antibodies with neutralizing activity
more efficiently in the whole immune course and the
later mucosal immunization improved this advantage.
MDCK cells pretreated with 3sM2 antisera showed
less pathogenicmorphological changes comparedwith
that of pretreated with sM2. Together, our results
demonstrated that high epitope density in one
recombinant protein can enhance humoral immune
responses in both systemic and mucosal immunization, and combination of systemic and mucosal
immunization enhances this advantage. |
