Title: Principal Investigator E-mail: zhouxi@wh.iov.cn


2001, B.S., College of Life Sciences, Wuhan University, Wuhan, China

2008, Ph.D., the Univ. of Texas MD Anderson Cancer Center, UTHealth Graduate School of Biomedical Sciences, Houston, TX, USA

2008-2011, Postdoctoral fellow, Dept. of Experimental Therapeutics, the Univ. of Texas MD Anderson Cancer Center, Houston, TX, USA

Research Interests

RNA viruses (flavivirus and enterovirus)

Virus interaction with antiviral immunity


1. Wang Z, Xia X, Yang X, Zhang X, Liu Y, Wu D, Fang Y, Liu Y, Qiu Y and Zhou X*. (2017) A Picorna-like Virus Suppresses the N-end Rule Pathway to Inhibit Apoptosis. eLife 2017;6:e30590 DOI: 10.7554/eLife.30590 .

2. Li T-F, Hosmillo M, Schwanke H, Shu T, Wang Z, Yin L, Curry S, Goodfellow IG and Zhou X*. (2017) Human Norovirus NS3 has RNA Helicase and Chaperoning Activities. J Virol. doi: 10.1128/JVI.01606-17

3. Qiu Y, Xu Y, Zhang Y, Zhou H, Deng Y-Q, Li X-F, Miao M, Zhang Q, Zhong B, Hu Y, Zhang F-C, Wu L, Qin C-F* and Zhou X*. (2017) Human Virus-Derived Small RNAs Can Confer Antiviral Immunity in Mammals. Immunity, 46(6): 992–1004.

4. Yang J, Qian Q, Li T-F, Yang X, Won S J, Zhou X*. (2017) Cypovirus capsid protein VP5 has nucleoside triphosphatase activity. Virol Sin, 32 (4): 328–330.

5. Wang Z, Wu D, Liu Y, Xia X, Gong W, Qiu Y, Yang J, Zheng Y, Li J, Wang YF, Xiang Y, Hu Y, Zhou X*. (2015) Drosophila Dicer-2 has an RNA interference-independent Function that Modulates Toll Immune Signaling. Science Advances, 1: e1500228.

6. Xia H, Wang P, Wang GC, Yang J, Sun X, Wu W, Qiu Y, Shu T, Zhao X, Yin L, Qin CF, Hu Y, Zhou X*. (2015) Human Enterovirus Nonstructural Protein 2CATPase Functions as Both an RNA Helicase and ATP-independent RNA Chaperone. PLoS Pathogens, 11(7): e1005067. doi: 10.1371/journal.ppat.1005067.

7.  Yang J, Xia H, Qian Q, Zhou X*, RNA chaperones encoded by RNA viruses, Virol Sin, 2015, 30(6): 401-409.

8. Yang J, Cheng Z, Zhang S, Xiong W, Xia H, Qiu Y, Wang Z, Wu F, Qin CF, Yin L, Hu Y and Zhou X* (2014) A Cypovirus VP5 Displays the RNA Chaperone-like Activity that Destabilizes RNA Helices and Accelerates Stranding Annealing. Nucleic Acids Res., 42(4): 2538-2554.

9. Qiu Y, Miao M, Wang Z, Liu Y, Yang J, Xia H, Li XF, Qin CF, Hu Y and Zhou X* (2014) The RNA Binding of Protein A from Wuhan Nodavirus is Mediated by Mitochondrial Membrane Lipids. Virology, 462-463: 1-13.

10. Tang F, Xia H, Wang P, Yang J, Zhao T, Zhang Q, Hu Y* and Zhou X*. (2014) The Identification and Characterization of Nucleic Acid Chaperone Activity of Human Enterovirus 71 nonstructural protein 3AB. Virology, 464-465: 353-364.

11. Qiu Y, Wang Z, Liu Y, Han Y, Miao M, Qi N, Yang J, Xia H, Li X, Qin CF, Hu Y* and Zhou X* (2014) The Self-interaction of a Nodavirus Replicase is Enhanced by Mitochondrial Membrane. PLoS ONE, 9(2): e89628. doi:10.1371/journal.pone.008962

12.  Wu W, Wang Z, Xia H, Liu Y, Qiu Y, Liu Y, Hu Y and Zhou X* (2014) Flock house virus RNA polymerase initiates RNA synthesis de novo and possesses a terminal nucleotidyl transferase activity. PLoS ONE 9(1): e86876. doi:10.1371/journal.pone.0086876

13. Zhao H, Jiang T, Zhou X-Z, Deng YQ, Li XF,  Chen SP, Zhu SY, Zhou X, Qin ED and Qin CF*. (2014) Induction of Neutralizing Antibodies against Four Serotypes of Dengue Viruses by MixBiEDIII,a Tetravalent Dengue Vaccine. PLoS ONE 9(1): e86573. doi:10.1371/journal.pone.0086573

14. Wang Z, Qiu Y, Liu Y, Qi N, Si J, Xia X, Wu D, Hu Y and Zhou X* (2013) Characterization of a Nodavirus Replicase Revealed a De Novo Initiation Mechanism of RNA Synthesis and Terminal Nucleotidyl Transferase Activity. J Biol Chem, 288(43): 30785-801.

15. Cheng Z, Yang J, Xia H, Qiu Y, Wang Z, Han Y, Xia X, Qin CF, Hu Y* and Zhou X* (2013) The Nonstructural Protein 2C of a Picorna-like Virus Displays Nucleic Acid Helix Destabilizing Activity that can be Functionally Separated from its ATPase Activity. J Virol. 87: 5205-18 (MDLinx Featured Article)

16. Qiu Y, Wang Z, Qi N, Si J, Liu Y, Xiang X, Hu Y and Zhou X*. (2013) Membrane association of Wuhan Nodavirus protein A is required for its ability to accumulate genomic RNA1 template. Virology. 439: 140-151 

17. Han Y, Wang Q, Qiu Y, Wu W, He H, Zhang J, Hu Y* and Zhou X* (2013) Periplaneta fuliginosa Densovirus Nonstructural Protein NS1 contains an endonuclease activity that is regulated by its phosphorylation. Virology, 437: 1-11.

18. Qiu Y*, Wang Z, Liu Y, Qi N, Si J, Xiang X, Xia X, Hu Y and Zhou X (2013) Newly discovered insect RNA viruses in China. Sci China Life Sci. 56(8):711-714

19. Liu ZY, Li XF, Jiang T, Deng YQ, Zhao H, Wang HJ, Ye Q, Zhu SY, Qiu Y, Zhou X, Qin ED, Qin CF (2013) Novel cis-acting element within the capsid-coding region enhances flavivirus viral-RNA replication by regulating genome cyclization. J Virol. 87:6804-18.

20. Qi N, Zhang L, Qiu Y, Wang Z, Si J, Liu Y, Xiang X, Xie J, Qin CF, Zhou X*, Hu Y (2012) Targeting of Dicer-2 and RNA by a Viral RNA Silencing Suppressor in Drosophila Cells. J Virol, 86:5763-5773. (J Virol Spotlight Article)

21. Wang Q, Han Y, Qiu Y, Zhang S, Tang F, Wang Y, Zhang J, Hu Y* and Zhou X* (2012) Identification and Characterization of RNA Duplex Unwinding and ATPase Activities of an Alphatetravirus Superfamily 1 Helicase. Virology, 433: 440-448.

22. Ye S., Xia H, Dong C, Cheng Z, Xia X, Zhang J, Zhou X*, and Hu Y* (2012) Identification and Characterization of Iflavirus 3C-like Protease Processing Activities. Virology, 428: 136-145.

23. Ye Q, Li XF, Zhao H, Li SH, Deng YQ, Cao RY, Song KY, Wang HJ, Hua RH, Yu YX, Zhou X, Qin ED, Qin CF. (2012) A single nucleotide mutation in NS2A of Japanese encephalitis-live vaccine virus (SA14-14-2) ablates NS1' formation and contributes to attenuation. J Gen Virol. 93:1959-64.

24. Qi N, Cai D, Qiu Y, Xie J, Wang Z, Si J, Zhang J, Zhou X* and Hu Y* (2011) RNA Binding by a Novel Helical Fold of B2 Protein from Wuhan Nodavirus Mediates RNAi Suppression and Promotes B2 Dimerization. J Virol, 85: 9543-9554.

25. Qiu Y, Cai D, Qi N, Wang Z, Zhou X, Zhang J and Hu Y (2011) Internal initiation is responsible for synthesis of Wuhan nodavirus subgenomic RNA. J Virol, 85: 4440-4451.

26. Zhou X, Si J, Corvera J, Gallick G, Kuang J. Decoding the Intrinsic Mechanism that Prohibits ALIX Interaction with ESCRT and Viral Proteins. (2010) Biochem J. 432(3):525-34.

27. Zhou X, Pan S, Sun L, Corvera J, Lee YC, Lin SH, Kuang J. (2009) The CHMP4b and Src docking sites in the Bro1 domain are autoinhibited in the native state of Alix. Biochem J. 418(2): 277-284.

28. Zhou X, Pan S, Sun L, Corvera J, Lin SH, Kuang J. (2008) The HIV-1 p6/EIAV p9 docking site in Alix is autoinhibited as revealed by a conformation sensitive anti-Alix monoclonal antibody. Biochem J. 414(2): 215-220.

29. Pan S, Wang R, Zhou X, Kloc M, Corvera J, Koomen J, Kobayashi R, Sifers R, Gallick GE, Lin SH and Kuang J. (2008) Extracellular Alix regulates integrin-mediated cell adhesions and extracellular matrix assembly. EMBO J. 27: 2077-2090.

30. Feng Z, Wu CF, Zhou X, Kuang J. Alternative polyadenylation produces two major transcripts of Alix. (2007) Arch Biochem Biophys 465: 328-335.

31. Pan S, Wang R, Zhou X, He G, Koomen J, Kobayashi R, Sun L, Corvera J, Gallick GE, Kuang J. (2006) Involvement of the conserved adaptor protein Alix in actin cytoskeleton assembly. J Biol Chem 281(45): 34640-34650.